Design Question 9

Six groups of oysters (3 male groups and 3 female groups) were selected from a clean site and six groups of oysters (3 male groups and 3 female groups) were selected from a polluted site in a tidal creek. (Oyster size is a reasonable proxy for gender). Six oysters from each group were sacrificed and morphological measurements were made--the response was an average morphological measurement from each group of a half-dozen oysters. Afterwards, the six groups of oysters from the clean site were transferred to the polluted site and the six groups of oysters from the polluted site were transfered to the clean site. After six weeks, six more oysters from each group were sacrificed and the measurements were repeated. Can you identify the design? Is there any serious confounding in the experiment? Can both gender and treatment (clean/polluted) effects be tested? How would you test them? Run the SAS code provided on the webpage and explain the results.